ssr_primer
Primer Details for TRINITY_DN60352_c0_g1_i2 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTGACATGCAGCTGATCCA | ATAGTTGTCTTGTGGCGGCA | Start: 308 |
| Tm | 60.107 | 59.964 | End: 541 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
|---|
| Sequence | TCCTGACATGCAGCTGATCC | ATAGTTGTCTTGTGGCGGCA | Start: 307 |
| Tm | 59.82 | 59.964 | End: 541 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
|---|
| Sequence | GCATCTCCTGACATGCAGCT | ATAGTTGTCTTGTGGCGGCA | Start: 302 |
| Tm | 60.464 | 59.964 | End: 541 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | TGCCGCCACAAGACAACTAT | TAGCAGCAATAACGGGAGCA | Start: 522 |
| Tm | 59.964 | 59.46 | End: 713 |
| Size | 20 | 20 | 192 |
| Primer 5 details |
|---|
| Sequence | TTTTGTTGCTATCGTGCCGC | TAGCAGCAATAACGGGAGCA | Start: 508 |
| Tm | 60.11 | 59.46 | End: 713 |
| Size | 20 | 20 | 206 |
(A)25: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTGACATGCAGCTGATCCA | ATAGTTGTCTTGTGGCGGCA | Start: 308 |
| Tm | 60.107 | 59.964 | End: 541 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
|---|
| Sequence | TCCTGACATGCAGCTGATCC | ATAGTTGTCTTGTGGCGGCA | Start: 307 |
| Tm | 59.82 | 59.964 | End: 541 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
|---|
| Sequence | GCATCTCCTGACATGCAGCT | ATAGTTGTCTTGTGGCGGCA | Start: 302 |
| Tm | 60.464 | 59.964 | End: 541 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | TGCCGCCACAAGACAACTAT | TAGCAGCAATAACGGGAGCA | Start: 522 |
| Tm | 59.964 | 59.46 | End: 713 |
| Size | 20 | 20 | 192 |
| Primer 5 details |
|---|
| Sequence | TTTTGTTGCTATCGTGCCGC | TAGCAGCAATAACGGGAGCA | Start: 508 |
| Tm | 60.11 | 59.46 | End: 713 |
| Size | 20 | 20 | 206 |
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