ssr_primer
Primer Details for TRINITY_DN60344_c0_g1_i1 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAAATCAAGCGATCCGGC | AATAAGGCCCACCACGAGTG | Start: 798 |
| Tm | 59.971 | 60.035 | End: 1019 |
| Size | 20 | 20 | 222 |
| Primer 2 details |
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| Sequence | GGAAAATCAAGCGATCCGGC | CAATAAGGCCCACCACGAGT | Start: 798 |
| Tm | 59.971 | 60.035 | End: 1020 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
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| Sequence | ACTCGTGGTGGGCCTTATTG | AGGCTGTTTCTTCGCGATGA | Start: 1001 |
| Tm | 60.035 | 60.037 | End: 1220 |
| Size | 20 | 20 | 220 |
| Primer 4 details |
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| Sequence | CACTCGTGGTGGGCCTTATT | AGGCTGTTTCTTCGCGATGA | Start: 1000 |
| Tm | 60.035 | 60.037 | End: 1220 |
| Size | 20 | 20 | 221 |
| Primer 5 details |
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| Sequence | CAAGGGTCGCATTCAGGACT | AGGCTGTTTCTTCGCGATGA | Start: 1063 |
| Tm | 60.036 | 60.037 | End: 1220 |
| Size | 20 | 20 | 158 |
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