ssr_primer
Primer Details for TRINITY_DN59823_c1_g1_i1 |
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| (A)34: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAGCTTTTTCGTCTCCGGC | AATCCAGGTTTGAGCCGAGG | Start: 129 |
| Tm | 59.834 | 60.035 | End: 285 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
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| Sequence | GAAGCTTTTTCGTCTCCGGC | GTCTCTTGCGCCTCCTGAAT | Start: 129 |
| Tm | 59.834 | 60.108 | End: 337 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
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| Sequence | GAAGCTTTTTCGTCTCCGGC | TCTCTTGCGCCTCCTGAATG | Start: 129 |
| Tm | 59.834 | 60.108 | End: 336 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
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| Sequence | CCTCGGCTCAAACCTGGATT | TAAGCCCTTGAACTGACCGC | Start: 266 |
| Tm | 60.035 | 60.321 | End: 499 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
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| Sequence | CCTCGGCTCAAACCTGGATT | ATTACTCGGATCGGGCCATG | Start: 266 |
| Tm | 60.035 | 59.678 | End: 463 |
| Size | 20 | 20 | 198 |
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