ssr_primer
Primer Details for TRINITY_DN59754_c0_g1_i1 |
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| (A)16tccaaattttccatgggaata(G)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATTGGGGGAAGGGGGAAAG | TGATCCCAACCCCTCGATCT | Start: 364 |
| Tm | 60.032 | 60.031 | End: 514 |
| Size | 20 | 20 | 151 |
| Primer 2 details |
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| Sequence | GATTGGGGGAAGGGGGAAAG | CCAGCCACTCAATGCCATTG | Start: 364 |
| Tm | 60.032 | 59.825 | End: 555 |
| Size | 20 | 20 | 192 |
| Primer 3 details |
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| Sequence | GATTGGGGGAAGGGGGAAAG | GTGGATGATCCCAACCCCTC | Start: 364 |
| Tm | 60.032 | 59.816 | End: 519 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
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| Sequence | GATTGGGGGAAGGGGGAAAG | AGTGGATGATCCCAACCCCT | Start: 364 |
| Tm | 60.032 | 60.254 | End: 520 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
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| Sequence | AGATCGAGGGGTTGGGATCA | GGAGACAGGAGGGAGGATCA | Start: 495 |
| Tm | 60.031 | 59.737 | End: 603 |
| Size | 20 | 20 | 109 |
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