ssr_primer
Primer Details for TRINITY_DN59662_c0_g1_i1 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGGTGGTCACATTTCCATG | AAACCACCGCACCTCTACAG | Start: 336 |
| Tm | 59.829 | 59.965 | End: 569 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
|---|
| Sequence | GGGCCGTGCTTTGAGAGATA | AAACCACCGCACCTCTACAG | Start: 443 |
| Tm | 59.822 | 59.965 | End: 569 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | TAACGGTGGTTTTGCGGAGA | CATGGAAATGTGACCACCGC | Start: 191 |
| Tm | 59.894 | 59.829 | End: 355 |
| Size | 20 | 20 | 165 |
| Primer 4 details |
|---|
| Sequence | TAACGGTGGTTTTGCGGAGA | TATCTCTCAAAGCACGGCCC | Start: 191 |
| Tm | 59.894 | 59.822 | End: 462 |
| Size | 20 | 20 | 272 |
| Primer 5 details |
|---|
| Sequence | TGCGGTGGTCACATTTCCAT | AAACCACCGCACCTCTACAG | Start: 335 |
| Tm | 60.251 | 59.965 | End: 569 |
| Size | 20 | 20 | 235 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGGTGGTCACATTTCCATG | AAACCACCGCACCTCTACAG | Start: 336 |
| Tm | 59.829 | 59.965 | End: 569 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
|---|
| Sequence | GGGCCGTGCTTTGAGAGATA | AAACCACCGCACCTCTACAG | Start: 443 |
| Tm | 59.822 | 59.965 | End: 569 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | TAACGGTGGTTTTGCGGAGA | CATGGAAATGTGACCACCGC | Start: 191 |
| Tm | 59.894 | 59.829 | End: 355 |
| Size | 20 | 20 | 165 |
| Primer 4 details |
|---|
| Sequence | TAACGGTGGTTTTGCGGAGA | TATCTCTCAAAGCACGGCCC | Start: 191 |
| Tm | 59.894 | 59.822 | End: 462 |
| Size | 20 | 20 | 272 |
| Primer 5 details |
|---|
| Sequence | TGCGGTGGTCACATTTCCAT | AAACCACCGCACCTCTACAG | Start: 335 |
| Tm | 60.251 | 59.965 | End: 569 |
| Size | 20 | 20 | 235 |
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