ssr_primer
Primer Details for TRINITY_DN59594_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCACCTCGGTTTCTGAGTC | CAGCACCCAGTTTCAACACG | Start: 257 |
| Tm | 60.037 | 59.971 | End: 439 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
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| Sequence | CCCACCTCGGTTTCTGAGTC | AGGTCCAGCGAGGGATTTTG | Start: 257 |
| Tm | 60.037 | 60.035 | End: 472 |
| Size | 20 | 20 | 216 |
| Primer 3 details |
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| Sequence | CCCACCTCGGTTTCTGAGTC | GGTCCAGCGAGGGATTTTGA | Start: 257 |
| Tm | 60.037 | 60.035 | End: 471 |
| Size | 20 | 20 | 215 |
| Primer 4 details |
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| Sequence | CCGCGATGATCTCTGTGACA | GACTCAGAAACCGAGGTGGG | Start: 42 |
| Tm | 59.899 | 60.037 | End: 276 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
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| Sequence | CCCACCTCGGTTTCTGAGTC | CTTTCAGAGGTCCAGCGAGG | Start: 257 |
| Tm | 60.037 | 60.108 | End: 479 |
| Size | 20 | 20 | 223 |
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