ssr_primer
Primer Details for TRINITY_DN59248_c0_g1_i3 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGACCAACTGGAGGAAAC | TACGCGACCACCAGAAAACA | Start: 445 |
| Tm | 59.892 | 59.896 | End: 600 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | TGTTTTCTGGTGGTCGCGTA | GTTTCCTCCAGTTGGTCCGT | Start: 336 |
| Tm | 59.896 | 59.892 | End: 464 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
|---|
| Sequence | CCCCGCTCACACACATGTAT | GTTTCCTCCAGTTGGTCCGT | Start: 307 |
| Tm | 60.108 | 59.892 | End: 464 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | AACTCTGATGCGTATGCCGT | ATACATGTGTGTGAGCGGGG | Start: 59 |
| Tm | 59.824 | 60.108 | End: 326 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | AGGGAAAACGGACCAACTGG | TACGCGACCACCAGAAAACA | Start: 438 |
| Tm | 60.179 | 59.896 | End: 600 |
| Size | 20 | 20 | 163 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGACCAACTGGAGGAAAC | TACGCGACCACCAGAAAACA | Start: 445 |
| Tm | 59.892 | 59.896 | End: 600 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | TGTTTTCTGGTGGTCGCGTA | GTTTCCTCCAGTTGGTCCGT | Start: 336 |
| Tm | 59.896 | 59.892 | End: 464 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
|---|
| Sequence | CCCCGCTCACACACATGTAT | GTTTCCTCCAGTTGGTCCGT | Start: 307 |
| Tm | 60.108 | 59.892 | End: 464 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | AACTCTGATGCGTATGCCGT | ATACATGTGTGTGAGCGGGG | Start: 59 |
| Tm | 59.824 | 60.108 | End: 326 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | AGGGAAAACGGACCAACTGG | TACGCGACCACCAGAAAACA | Start: 438 |
| Tm | 60.179 | 59.896 | End: 600 |
| Size | 20 | 20 | 163 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGACCAACTGGAGGAAAC | TACGCGACCACCAGAAAACA | Start: 445 |
| Tm | 59.892 | 59.896 | End: 600 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | TGTTTTCTGGTGGTCGCGTA | GTTTCCTCCAGTTGGTCCGT | Start: 336 |
| Tm | 59.896 | 59.892 | End: 464 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
|---|
| Sequence | CCCCGCTCACACACATGTAT | GTTTCCTCCAGTTGGTCCGT | Start: 307 |
| Tm | 60.108 | 59.892 | End: 464 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | AACTCTGATGCGTATGCCGT | ATACATGTGTGTGAGCGGGG | Start: 59 |
| Tm | 59.824 | 60.108 | End: 326 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | AGGGAAAACGGACCAACTGG | TACGCGACCACCAGAAAACA | Start: 438 |
| Tm | 60.179 | 59.896 | End: 600 |
| Size | 20 | 20 | 163 |
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