ssr_primer
Primer Details for TRINITY_DN58853_c0_g1_i1 |
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| (T)28ggagggttgatttagggccgcgcgcgggtgggt(G)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAATCTCAGCCGGACAGGTG | CGGAGGATCGAGACTCAACG | Start: 266 |
| Tm | 60.036 | 59.972 | End: 395 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
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| Sequence | CGTTGAGTCTCGATCCTCCG | CTCTGAGAAAGCGGCCAGAA | Start: 376 |
| Tm | 59.972 | 60.037 | End: 514 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
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| Sequence | AAATCTCAGCCGGACAGGTG | CTCTGAGAAAGCGGCCAGAA | Start: 266 |
| Tm | 60.036 | 60.037 | End: 514 |
| Size | 20 | 20 | 249 |
| Primer 4 details |
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| Sequence | CGGACAGGTGATCGTTAGGG | CGGAGGATCGAGACTCAACG | Start: 276 |
| Tm | 59.898 | 59.972 | End: 395 |
| Size | 20 | 20 | 120 |
| Primer 5 details |
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| Sequence | CCGGACAGGTGATCGTTAGG | CGGAGGATCGAGACTCAACG | Start: 275 |
| Tm | 59.898 | 59.972 | End: 395 |
| Size | 20 | 20 | 121 |
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