ssr_primer
Primer Details for TRINITY_DN58263_c0_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAACTACGTACATTAGCCCG | TAAAAAGGGGCGGAGGGTTC | Start: 61 |
| Tm | 59.9 | 59.961 | End: 188 |
| Size | 22 | 20 | 128 |
| Primer 2 details |
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| Sequence | TGGAACTACGTACATTAGCCCG | CTTAAAAAGGGGCGGAGGGT | Start: 61 |
| Tm | 59.9 | 59.961 | End: 190 |
| Size | 22 | 20 | 130 |
| Primer 3 details |
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| Sequence | ACGTACATTAGCCCGAATACCG | TAAAAAGGGGCGGAGGGTTC | Start: 68 |
| Tm | 60.288 | 59.961 | End: 188 |
| Size | 22 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | ACGTACATTAGCCCGAATACCG | CTTAAAAAGGGGCGGAGGGT | Start: 68 |
| Tm | 60.288 | 59.961 | End: 190 |
| Size | 22 | 20 | 123 |
| Primer 5 details |
|---|
| Sequence | CGTACATTAGCCCGAATACCG | TAAAAAGGGGCGGAGGGTTC | Start: 69 |
| Tm | 58.678 | 59.961 | End: 188 |
| Size | 21 | 20 | 120 |
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