Primer Details for TRINITY_DN58075_c0_g1_i1 | |||
|---|---|---|---|
| (A)30: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTGAGAAAAGTTAGCTCCCA | CGTGTTTGAAGTTTCGAAATCACA | Start: 98 |
| Tm | 59.628 | 58.975 | End: 203 |
| Size | 22 | 24 | 106 |
| Primer 2 details | |||
| Sequence | TGCTGAGAAAAGTTAGCTCCCA | ACGTGTTTGAAGTTTCGAAATCAC | Start: 98 |
| Tm | 59.628 | 58.974 | End: 204 |
| Size | 22 | 24 | 107 |
| Primer 3 details | |||
| Sequence | TGCTGAGAAAAGTTAGCTCCCA | ACGTGTTTGAAGTTTCGAAATCA | Start: 98 |
| Tm | 59.628 | 57.775 | End: 204 |
| Size | 22 | 23 | 107 |
| Primer 4 details | |||
| Sequence | TGCTGAGAAAAGTTAGCTCCCA | ACGTGTTTGAAGTTTCGAAATCACA | Start: 98 |
| Tm | 59.628 | 60.391 | End: 204 |
| Size | 22 | 25 | 107 |
| Primer 5 details | |||
| Sequence | TGCTGAGAAAAGTTAGCTCCCA | CGTGTTTGAAGTTTCGAAATCAC | Start: 98 |
| Tm | 59.628 | 57.474 | End: 203 |
| Size | 22 | 23 | 106 |
