ssr_primer
Primer Details for TRINITY_DN57273_c0_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGTTCTTCGCTACACGTT | TGTTATGGGTTGCCTGTCCC | Start: 601 |
| Tm | 59.969 | 59.961 | End: 859 |
| Size | 20 | 20 | 259 |
| Primer 2 details |
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| Sequence | TAAAGCCGCGTCACAGTGAT | TGTTATGGGTTGCCTGTCCC | Start: 668 |
| Tm | 60.038 | 59.961 | End: 859 |
| Size | 20 | 20 | 192 |
| Primer 3 details |
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| Sequence | TTTCATCGCCGAGAAGGACC | ATCACTGTGACGCGGCTTTA | Start: 584 |
| Tm | 60.108 | 60.038 | End: 687 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
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| Sequence | TTTCATCGCCGAGAAGGACC | TGTTATGGGTTGCCTGTCCC | Start: 584 |
| Tm | 60.108 | 59.961 | End: 859 |
| Size | 20 | 20 | 276 |
| Primer 5 details |
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| Sequence | AGGACCGTTCTTCGCTACAC | TGTTATGGGTTGCCTGTCCC | Start: 598 |
| Tm | 59.757 | 59.961 | End: 859 |
| Size | 20 | 20 | 262 |
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