ssr_primer
Primer Details for TRINITY_DN56827_c0_g1_i3 |
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| (A)33: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGTCGGATAATGGACAGC | GGATGAGCGTTAAGTGGCCT | Start: 102 |
| Tm | 59.896 | 60.108 | End: 213 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
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| Sequence | ACCGTCGGATAATGGACAGC | TGGATGAGCGTTAAGTGGCC | Start: 102 |
| Tm | 59.896 | 60.392 | End: 214 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
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| Sequence | CCGTCGGATAATGGACAGCT | GGATGAGCGTTAAGTGGCCT | Start: 103 |
| Tm | 59.611 | 60.108 | End: 213 |
| Size | 20 | 20 | 111 |
| Primer 4 details |
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| Sequence | AGGCCACTTAACGCTCATCC | GCCCATCGACTGGCCATTAA | Start: 194 |
| Tm | 60.108 | 60.465 | End: 469 |
| Size | 20 | 20 | 276 |
| Primer 5 details |
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| Sequence | AATAGACAGGACTCGGTCGC | GGGAGGAGAAAAGGCGTTGA | Start: 24 |
| Tm | 59.26 | 59.964 | End: 187 |
| Size | 20 | 20 | 164 |
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