ssr_primer
Primer Details for TRINITY_DN55529_c0_g1_i1 |
|---|
| (AAT)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTTACTTTGGCCACCCCC | GCTACATCTTCTCCTCCGGC | Start: 290 |
| Tm | 59.886 | 59.967 | End: 420 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
|---|
| Sequence | TTCTTACTTTGGCCACCCCC | GAAGCGACTTCGTGGATTGC | Start: 290 |
| Tm | 59.886 | 59.905 | End: 448 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | TTCTTACTTTGGCCACCCCC | CCAGAAGCGACTTCGTGGAT | Start: 290 |
| Tm | 59.886 | 60.109 | End: 451 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | CCGCTTCTGGAAACGCAATT | GCTACATCTTCTCCTCCGGC | Start: 212 |
| Tm | 59.759 | 59.967 | End: 420 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | ATTTGCCGCTTCTGGAAACG | GCTACATCTTCTCCTCCGGC | Start: 207 |
| Tm | 59.759 | 59.967 | End: 420 |
| Size | 20 | 20 | 214 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTTACTTTGGCCACCCCC | GCTACATCTTCTCCTCCGGC | Start: 290 |
| Tm | 59.886 | 59.967 | End: 420 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
|---|
| Sequence | TTCTTACTTTGGCCACCCCC | GAAGCGACTTCGTGGATTGC | Start: 290 |
| Tm | 59.886 | 59.905 | End: 448 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | TTCTTACTTTGGCCACCCCC | CCAGAAGCGACTTCGTGGAT | Start: 290 |
| Tm | 59.886 | 60.109 | End: 451 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | CCGCTTCTGGAAACGCAATT | GCTACATCTTCTCCTCCGGC | Start: 212 |
| Tm | 59.759 | 59.967 | End: 420 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | ATTTGCCGCTTCTGGAAACG | GCTACATCTTCTCCTCCGGC | Start: 207 |
| Tm | 59.759 | 59.967 | End: 420 |
| Size | 20 | 20 | 214 |
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