ssr_primer
Primer Details for TRINITY_DN54543_c0_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAACTCAAGTGCAGTGGGGT | GGCAAGGGCGTTTAAACCTC | Start: 325 |
| Tm | 60.179 | 59.757 | End: 490 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
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| Sequence | TGAACTCAAGTGCAGTGGGG | GGCAAGGGCGTTTAAACCTC | Start: 324 |
| Tm | 60.179 | 59.757 | End: 490 |
| Size | 20 | 20 | 167 |
| Primer 3 details |
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| Sequence | GGAACGCATGATTGCCTTCG | ACCCCACTGCACTTGAGTTC | Start: 70 |
| Tm | 60.248 | 60.179 | End: 344 |
| Size | 20 | 20 | 275 |
| Primer 4 details |
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| Sequence | GGAACGCATGATTGCCTTCG | CCCCACTGCACTTGAGTTCA | Start: 70 |
| Tm | 60.248 | 60.179 | End: 343 |
| Size | 20 | 20 | 274 |
| Primer 5 details |
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| Sequence | AACTCAAGTGCAGTGGGGTG | GGCAAGGGCGTTTAAACCTC | Start: 326 |
| Tm | 60.466 | 59.757 | End: 490 |
| Size | 20 | 20 | 165 |
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