ssr_primer
Primer Details for TRINITY_DN54429_c0_g1_i1 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAATTGACCTCCCGGGACCT | CGGGGGTACAAAGGGAGTTC | Start: 568 |
| Tm | 59.956 | 60.036 | End: 758 |
| Size | 20 | 20 | 191 |
| Primer 2 details |
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| Sequence | GAAAGTTGATGAACGGCCGG | CGGGGGTACAAAGGGAGTTC | Start: 546 |
| Tm | 59.832 | 60.036 | End: 758 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
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| Sequence | GTTGAGGAAACGAGGCGGTA | CATCGACTCACTCCGTTCGT | Start: 228 |
| Tm | 60.038 | 59.832 | End: 348 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
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| Sequence | GTTGAGGAAACGAGGCGGTA | ACATCGACTCACTCCGTTCG | Start: 228 |
| Tm | 60.038 | 59.832 | End: 349 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
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| Sequence | CGAACGGAGTGAGTCGATGT | TCAATCTTTTCGCCGGTCGA | Start: 330 |
| Tm | 59.832 | 60.038 | End: 547 |
| Size | 20 | 20 | 218 |
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