ssr_primer
Primer Details for TRINITY_DN53568_c0_g1_i1 |
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| (C)33gccgcgggacctaatgtttcggccggccccttccccctcctttta(T)10cttgtccaatttttttgggcgggcg(T)28: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGTTAGTCCTGGGGCGATC | TAAAAGGAGGGGGAAGGGGC | Start: 61 |
| Tm | 60.179 | 61.219 | End: 163 |
| Size | 20 | 20 | 103 |
| Primer 2 details |
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| Sequence | GTGTTAGTCCTGGGGCGATC | AAAAACGCCCGCCCAAAAA | Start: 61 |
| Tm | 60.179 | 59.779 | End: 203 |
| Size | 20 | 19 | 143 |
| Primer 3 details |
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| Sequence | GTGTTAGTCCTGGGGCGATC | ATAAAAGGAGGGGGAAGGGG | Start: 61 |
| Tm | 60.179 | 58.379 | End: 164 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
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| Sequence | TTGTGGTGTTAGTCCTGGGG | TAAAAGGAGGGGGAAGGGGC | Start: 56 |
| Tm | 59.231 | 61.219 | End: 163 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
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| Sequence | TTGTGGTGTTAGTCCTGGGG | AAAAACGCCCGCCCAAAAA | Start: 56 |
| Tm | 59.231 | 59.779 | End: 203 |
| Size | 20 | 19 | 148 |
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