ssr_primer
Primer Details for TRINITY_DN53353_c0_g1_i1 |
|---|
| (T)37: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTGTTGGGTGCAGAAACT | AACGCTCTGACCATTAGGGC | Start: 491 |
| Tm | 60.034 | 60.108 | End: 675 |
| Size | 20 | 20 | 185 |
| Primer 2 details |
|---|
| Sequence | CCGGGCGTTGGAAAACATTT | TTCTACGCACCCGGCTTTAG | Start: 44 |
| Tm | 59.967 | 60.109 | End: 257 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | CCGGGCGTTGGAAAACATTT | GACAGGGAGTTCTACGCACC | Start: 44 |
| Tm | 59.967 | 60.109 | End: 266 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
|---|
| Sequence | CCGGGCGTTGGAAAACATTT | GGACAGGGAGTTCTACGCAC | Start: 44 |
| Tm | 59.967 | 60.109 | End: 267 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | GTGCGTAGAACTCCCTGTCC | AGTTTCTGCACCCAACACCA | Start: 248 |
| Tm | 60.109 | 60.034 | End: 510 |
| Size | 20 | 20 | 263 |
(G)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTGTTGGGTGCAGAAACT | AACGCTCTGACCATTAGGGC | Start: 491 |
| Tm | 60.034 | 60.108 | End: 675 |
| Size | 20 | 20 | 185 |
| Primer 2 details |
|---|
| Sequence | CCGGGCGTTGGAAAACATTT | TTCTACGCACCCGGCTTTAG | Start: 44 |
| Tm | 59.967 | 60.109 | End: 257 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | CCGGGCGTTGGAAAACATTT | GACAGGGAGTTCTACGCACC | Start: 44 |
| Tm | 59.967 | 60.109 | End: 266 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
|---|
| Sequence | CCGGGCGTTGGAAAACATTT | GGACAGGGAGTTCTACGCAC | Start: 44 |
| Tm | 59.967 | 60.109 | End: 267 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | GTGCGTAGAACTCCCTGTCC | AGTTTCTGCACCCAACACCA | Start: 248 |
| Tm | 60.109 | 60.034 | End: 510 |
| Size | 20 | 20 | 263 |
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