ssr_primer
Primer Details for TRINITY_DN51189_c0_g2_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGAAGGGGCTAGATCGGCT | TCCCCTTATTTGGACACGCA | Start: 47 |
| Tm | 60.399 | 59.306 | End: 184 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
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| Sequence | GGGGCTAGATCGGCTCACTA | TCCCCTTATTTGGACACGCA | Start: 52 |
| Tm | 60.54 | 59.306 | End: 184 |
| Size | 20 | 20 | 133 |
| Primer 3 details |
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| Sequence | AAGAAGGGGCTAGATCGGCT | TGGACACGCAGATTCGTTTG | Start: 47 |
| Tm | 60.399 | 59.135 | End: 174 |
| Size | 20 | 20 | 128 |
| Primer 4 details |
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| Sequence | GGGGCTAGATCGGCTCACTA | TGGACACGCAGATTCGTTTG | Start: 52 |
| Tm | 60.54 | 59.135 | End: 174 |
| Size | 20 | 20 | 123 |
| Primer 5 details |
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| Sequence | AACTGGCTTCAGGCGTTGAT | TCTCTTTTCCTCCCTTCCCCT | Start: 91 |
| Tm | 60.251 | 59.846 | End: 199 |
| Size | 20 | 21 | 109 |
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