ssr_primer
Primer Details for TRINITY_DN51178_c0_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCCTTTGCTAGGCGAGTAA | GTTCGACTCGTGGCCAAAAC | Start: 475 |
| Tm | 60.108 | 60.042 | End: 731 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
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| Sequence | ATCCCAAACCGAACAGGCAA | GTTCGACTCGTGGCCAAAAC | Start: 628 |
| Tm | 60.179 | 60.042 | End: 731 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
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| Sequence | GCCCTTTGCTAGGCGAGTAA | TTGCCTGTTCGGTTTGGGAT | Start: 475 |
| Tm | 60.108 | 60.179 | End: 647 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
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| Sequence | GCCCTTTGCTAGGCGAGTAA | ACGTCTTGTTCGACTCGTGG | Start: 475 |
| Tm | 60.108 | 60.318 | End: 738 |
| Size | 20 | 20 | 264 |
| Primer 5 details |
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| Sequence | ATGTGGCGAATCTCAGCGTT | GTTCGACTCGTGGCCAAAAC | Start: 547 |
| Tm | 60.392 | 60.042 | End: 731 |
| Size | 20 | 20 | 185 |
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