ssr_primer
Primer Details for TRINITY_DN50868_c0_g1_i1 |
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| (TGC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACAGAAGTTGCTGCTGCTG | CCGTTCTGGAAGCAGAAGAGA | Start: 176 |
| Tm | 60.04 | 59.726 | End: 296 |
| Size | 20 | 21 | 121 |
| Primer 2 details |
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| Sequence | CACAGAAGTTGCTGCTGCTG | TCCGTTCTGGAAGCAGAAGAG | Start: 176 |
| Tm | 60.04 | 59.726 | End: 297 |
| Size | 20 | 21 | 122 |
| Primer 3 details |
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| Sequence | TGTGCTGTTGTTTCGGATGTG | CAGCAGCAGCAACTTCTGTG | Start: 73 |
| Tm | 59.667 | 60.04 | End: 195 |
| Size | 21 | 20 | 123 |
| Primer 4 details |
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| Sequence | GTGCTGTTGTTTCGGATGTGT | CAGCAGCAGCAACTTCTGTG | Start: 74 |
| Tm | 59.666 | 60.04 | End: 195 |
| Size | 21 | 20 | 122 |
| Primer 5 details |
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| Sequence | CACAGAAGTTGCTGCTGCTG | TCCGTTCTGGAAGCAGAAGA | Start: 176 |
| Tm | 60.04 | 58.662 | End: 297 |
| Size | 20 | 20 | 122 |
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