ssr_primer
Primer Details for TRINITY_DN50758_c0_g1_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGAACCTTCGTGAAACCA | AGCGAGTCTCAGATTTGCCC | Start: 208 |
| Tm | 59.821 | 60.108 | End: 454 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
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| Sequence | TTGCTGACTTCGGTGAGTGG | TGGTTTCACGAAGGTTCCGT | Start: 11 |
| Tm | 60.25 | 59.821 | End: 227 |
| Size | 20 | 20 | 217 |
| Primer 3 details |
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| Sequence | CGGTGAGTGGTCTGAAGGTG | TGGTTTCACGAAGGTTCCGT | Start: 21 |
| Tm | 60.32 | 59.821 | End: 227 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
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| Sequence | TCGGTGAGTGGTCTGAAGGT | TGGTTTCACGAAGGTTCCGT | Start: 20 |
| Tm | 60.469 | 59.821 | End: 227 |
| Size | 20 | 20 | 208 |
| Primer 5 details |
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| Sequence | ACGGAACCTTCGTGAAACCA | CGAGTCTCAGATTTGCCCCT | Start: 208 |
| Tm | 59.821 | 59.461 | End: 452 |
| Size | 20 | 20 | 245 |
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