Primer Details for TRINITY_DN45608_c0_g1_i1 | |||
|---|---|---|---|
| (T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTGTAACTCAATGTAAGGTTTT | TCTCTCAATAAGTTCGTGCAGA | Start: 80 |
| Tm | 57.069 | 57.216 | End: 180 |
| Size | 24 | 22 | 101 |
| Primer 2 details | |||
| Sequence | AACGTGTAACTCAATGTAAGGTTT | TCTCTCAATAAGTTCGTGCAGA | Start: 79 |
| Tm | 57.069 | 57.216 | End: 180 |
| Size | 24 | 22 | 102 |
| Primer 3 details | |||
| Sequence | ACAAGAGTCAAATAACGTGTAACTCA | TCTCTCAATAAGTTCGTGCAGA | Start: 66 |
| Tm | 59.018 | 57.216 | End: 180 |
| Size | 26 | 22 | 115 |
| Primer 4 details | |||
| Sequence | GACAAGAGTCAAATAACGTGTAACTCA | TCTCTCAATAAGTTCGTGCAGA | Start: 65 |
| Tm | 59.831 | 57.216 | End: 180 |
| Size | 27 | 22 | 116 |
| Primer 5 details | |||
| Sequence | ACGTGTAACTCAATGTAAGGTTTT | TCTCTCAATAAGTTCGTGCAGAA | Start: 80 |
| Tm | 57.069 | 57.866 | End: 180 |
| Size | 24 | 23 | 101 |
