Primer Details for TRINITY_DN4550_c0_g1_i1 |
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| (A)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCAGTTCAACTGCCGTGCT | TGAAAAGTCCAAGGAAGAATATGCA | Start: 44 |
| Tm | 60.25 | 58.99 | End: 161 |
| Size | 20 | 25 | 118 |
| Primer 2 details |
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| Sequence | ATCAGTTCAACTGCCGTGCT | CCAAGGAAGAATATGCATAACTGTCC | Start: 44 |
| Tm | 60.25 | 60.017 | End: 153 |
| Size | 20 | 26 | 110 |
| Primer 3 details |
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| Sequence | ATCAGTTCAACTGCCGTGCT | AGGAAGAATATGCATAACTGTCCTGA | Start: 44 |
| Tm | 60.25 | 59.9 | End: 150 |
| Size | 20 | 26 | 107 |
| Primer 4 details |
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| Sequence | TCAGTTCAACTGCCGTGCTA | TGAAAAGTCCAAGGAAGAATATGCA | Start: 45 |
| Tm | 59.609 | 58.99 | End: 161 |
| Size | 20 | 25 | 117 |
| Primer 5 details |
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| Sequence | ATCAGTTCAACTGCCGTGCT | CTGAAAAGTCCAAGGAAGAATATGCA | Start: 44 |
| Tm | 60.25 | 59.848 | End: 162 |
| Size | 20 | 26 | 119 |
