ssr_primer
Primer Details for TRINITY_DN45464_c0_g1_i1 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCCGGCTCTCTTATTAGG | AGACGGGAGGGAGGCTAAAT | Start: 481 |
| Tm | 60.038 | 60.031 | End: 695 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
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| Sequence | CCATTCTCAAAGCCCCGTCT | CCTAATAAGAGAGCCGGCGG | Start: 356 |
| Tm | 60.035 | 60.038 | End: 500 |
| Size | 20 | 20 | 145 |
| Primer 3 details |
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| Sequence | GTCCTTCCTTTCTCTCCGCC | AGACGGGAGGGAGGCTAAAT | Start: 500 |
| Tm | 60.108 | 60.031 | End: 695 |
| Size | 20 | 20 | 196 |
| Primer 4 details |
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| Sequence | GGTCCTTCCTTTCTCTCCGC | AGACGGGAGGGAGGCTAAAT | Start: 499 |
| Tm | 60.108 | 60.031 | End: 695 |
| Size | 20 | 20 | 197 |
| Primer 5 details |
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| Sequence | CCATTCTCAAAGCCCCGTCT | GCGGAGAGAAAGGAAGGACC | Start: 356 |
| Tm | 60.035 | 60.108 | End: 518 |
| Size | 20 | 20 | 163 |
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