Primer Details for TRINITY_DN29730_c0_g1_i1 |
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| (C)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCAACTTCAGAATTCAATTGCT | CGTTGTTCGAGTACGGTCGT | Start: 12 |
| Tm | 57.111 | 60.385 | End: 130 |
| Size | 24 | 20 | 119 |
| Primer 2 details |
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| Sequence | TTCTCAACTTCAGAATTCAATTGCT | CGTTGTTCGAGTACGGTCGT | Start: 11 |
| Tm | 57.719 | 60.385 | End: 130 |
| Size | 25 | 20 | 120 |
| Primer 3 details |
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| Sequence | TCTCAACTTCAGAATTCAATTGCT | CGTTGTTCGAGTACGGTCGTA | Start: 12 |
| Tm | 57.111 | 60.136 | End: 130 |
| Size | 24 | 21 | 119 |
| Primer 4 details |
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| Sequence | ATTCTCAACTTCAGAATTCAATTGCT | CGTTGTTCGAGTACGGTCGT | Start: 10 |
| Tm | 58.106 | 60.385 | End: 130 |
| Size | 26 | 20 | 121 |
| Primer 5 details |
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| Sequence | TCTCAACTTCAGAATTCAATTGCTA | CGTTGTTCGAGTACGGTCGT | Start: 12 |
| Tm | 57.016 | 60.385 | End: 130 |
| Size | 25 | 20 | 119 |
