Primer Details for TRINITY_DN29449_c0_g1_i1 | |||
|---|---|---|---|
| (G)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGGCACCGTTGATTAAGA | TGCAGGGATTATGATGATTTCACT | Start: 38 |
| Tm | 59.678 | 58.302 | End: 177 |
| Size | 20 | 24 | 140 |
| Primer 2 details | |||
| Sequence | GGGGAGGGCATTTTTAGGCA | TGCAGGGATTATGATGATTTCACT | Start: 77 |
| Tm | 60.325 | 58.302 | End: 177 |
| Size | 20 | 24 | 101 |
| Primer 3 details | |||
| Sequence | ACCGGCACCGTTGATTAAGA | AGTTCTATTTGGAATTTTGCAGGGA | Start: 38 |
| Tm | 59.678 | 59.22 | End: 194 |
| Size | 20 | 25 | 157 |
| Primer 4 details | |||
| Sequence | GGGGAGGGCATTTTTAGGCA | AGTTCTATTTGGAATTTTGCAGGGA | Start: 77 |
| Tm | 60.325 | 59.22 | End: 194 |
| Size | 20 | 25 | 118 |
| Primer 5 details | |||
| Sequence | CACCGGCACCGTTGATTAAG | TGCAGGGATTATGATGATTTCACT | Start: 37 |
| Tm | 59.554 | 58.302 | End: 177 |
| Size | 20 | 24 | 141 |
