ssr_primer
Primer Details for TRINITY_DN2425_c0_g1_i1 |
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| (T)10gaaaattgtttgacccccagttccggttttttcccgcctcctcccatcgatccggtccggtg(T)31: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTTTGACCCCCAGTTCCG | GCAGGTCGCAATTTCAGGTC | Start: 24 |
| Tm | 60.467 | 59.831 | End: 216 |
| Size | 20 | 20 | 193 |
| Primer 2 details |
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| Sequence | GACCCCCAGTTCCGGTTTTT | GCAGGTCGCAATTTCAGGTC | Start: 30 |
| Tm | 60.469 | 59.831 | End: 216 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
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| Sequence | TTGTTTGACCCCCAGTTCCG | CAGGTCTCGGATGAGCTTCG | Start: 24 |
| Tm | 60.467 | 60.249 | End: 202 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
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| Sequence | GACCCCCAGTTCCGGTTTTT | CAGGTCTCGGATGAGCTTCG | Start: 30 |
| Tm | 60.469 | 60.249 | End: 202 |
| Size | 20 | 20 | 173 |
| Primer 5 details |
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| Sequence | TTGTTTGACCCCCAGTTCCG | GCACACTGGACCGTAGTTCT | Start: 24 |
| Tm | 60.467 | 59.683 | End: 142 |
| Size | 20 | 20 | 119 |
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