ssr_primer
Primer Details for TRINITY_DN2301_c0_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGGGCGATTGACAAGTCT | TGGTCGTGGTTAGCGAGTTT | Start: 133 |
| Tm | 59.023 | 59.61 | End: 253 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
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| Sequence | TCAACTTCTGGGCGATTGACA | TGGTCGTGGTTAGCGAGTTT | Start: 127 |
| Tm | 59.928 | 59.61 | End: 253 |
| Size | 21 | 20 | 127 |
| Primer 3 details |
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| Sequence | ACTTCTGGGCGATTGACAAGT | TGGTCGTGGTTAGCGAGTTT | Start: 130 |
| Tm | 59.928 | 59.61 | End: 253 |
| Size | 21 | 20 | 124 |
| Primer 4 details |
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| Sequence | TCTGGGCGATTGACAAGTCT | GGAGATTCTCAATCGCCGCA | Start: 133 |
| Tm | 59.023 | 60.531 | End: 284 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
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| Sequence | TCTGGGCGATTGACAAGTCT | ATGGTCGTGGTTAGCGAGTT | Start: 133 |
| Tm | 59.023 | 59.393 | End: 254 |
| Size | 20 | 20 | 122 |
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