Primer Details for TRINITY_DN21711_c0_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGTGAAACGATATAAATGAGCA | TGTACCGTTGTTTGTGCCCA | Start: 64 |
| Tm | 58.319 | 60.394 | End: 186 |
| Size | 24 | 20 | 123 |
| Primer 2 details |
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| Sequence | GAGGGTGAAACGATATAAATGAGCA | TGTACCGTTGTTTGTGCCCA | Start: 63 |
| Tm | 59.242 | 60.394 | End: 186 |
| Size | 25 | 20 | 124 |
| Primer 3 details |
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| Sequence | AAAAGAAAAAGAGGGTGAAACGA | TGTACCGTTGTTTGTGCCCA | Start: 53 |
| Tm | 57.092 | 60.394 | End: 186 |
| Size | 23 | 20 | 134 |
| Primer 4 details |
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| Sequence | AGAGGGTGAAACGATATAAATGAGC | TGTACCGTTGTTTGTGCCCA | Start: 62 |
| Tm | 59.008 | 60.394 | End: 186 |
| Size | 25 | 20 | 125 |
| Primer 5 details |
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| Sequence | AGGGTGAAACGATATAAATGAGCAA | TGTACCGTTGTTTGTGCCCA | Start: 64 |
| Tm | 58.88 | 60.394 | End: 186 |
| Size | 25 | 20 | 123 |
