Primer Details for TRINITY_DN19049_c0_g1_i1 |
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| (A)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGAATGAATGGGTCAGTTGCG | AAGTTGCTGTTTAGACGGATCT | Start: 78 |
| Tm | 59.771 | 57.727 | End: 177 |
| Size | 22 | 22 | 100 |
| Primer 2 details |
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| Sequence | ATGAATGGGTCAGTTGCGCT | TGAAATTAAGTTGCTGTTTAGACGGA | Start: 82 |
| Tm | 60.323 | 59.516 | End: 184 |
| Size | 20 | 26 | 103 |
| Primer 3 details |
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| Sequence | TTGAATGAATGGGTCAGTTGCG | AAGTTGCTGTTTAGACGGATCTT | Start: 78 |
| Tm | 59.771 | 58.358 | End: 177 |
| Size | 22 | 23 | 100 |
| Primer 4 details |
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| Sequence | ATGAATGGGTCAGTTGCGCT | GTGAAATTAAGTTGCTGTTTAGACGG | Start: 82 |
| Tm | 60.323 | 59.423 | End: 185 |
| Size | 20 | 26 | 104 |
| Primer 5 details |
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| Sequence | AATGAATGGGTCAGTTGCGC | ATTAAGTTGCTGTTTAGACGGATCT | Start: 81 |
| Tm | 59.472 | 58.593 | End: 180 |
| Size | 20 | 25 | 100 |
