Primer Details for TRINITY_DN168247_c0_g1_i1 |
|---|
| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAAGTCTAACAGAAACGCATAACA | AGTTAAGGTCTTTTCTTATGCTTCGA | Start: 68 |
| Tm | 59.296 | 58.841 | End: 179 |
| Size | 26 | 26 | 112 |
| Primer 2 details |
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| Sequence | AGTCTAACAGAAACGCATAACAAA | AGTTAAGGTCTTTTCTTATGCTTCGA | Start: 72 |
| Tm | 57.146 | 58.841 | End: 179 |
| Size | 24 | 26 | 108 |
| Primer 3 details |
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| Sequence | AAGTCTAACAGAAACGCATAACAA | AGTTAAGGTCTTTTCTTATGCTTCGA | Start: 71 |
| Tm | 57.146 | 58.841 | End: 179 |
| Size | 24 | 26 | 109 |
| Primer 4 details |
|---|
| Sequence | AAAGTCTAACAGAAACGCATAACA | AGTTAAGGTCTTTTCTTATGCTTCGA | Start: 70 |
| Tm | 57.146 | 58.841 | End: 179 |
| Size | 24 | 26 | 110 |
| Primer 5 details |
|---|
| Sequence | GAAAGTCTAACAGAAACGCATAACA | AGTTAAGGTCTTTTCTTATGCTTCGA | Start: 69 |
| Tm | 58.116 | 58.841 | End: 179 |
| Size | 25 | 26 | 111 |
