Primer Details for TRINITY_DN166853_c0_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTTTGAGATAGCAGATATTCAGT | ACCTTATGAATTCTAGCAGACTCA | Start: 31 |
| Tm | 57.628 | 57.202 | End: 170 |
| Size | 25 | 24 | 140 |
| Primer 2 details |
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| Sequence | AGGTTTGAGATAGCAGATATTCAGT | AACCTTATGAATTCTAGCAGACTCA | Start: 31 |
| Tm | 57.628 | 57.812 | End: 171 |
| Size | 25 | 25 | 141 |
| Primer 3 details |
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| Sequence | AAGGTTTGAGATAGCAGATATTCAGT | ACCTTATGAATTCTAGCAGACTCA | Start: 30 |
| Tm | 58.2 | 57.202 | End: 170 |
| Size | 26 | 24 | 141 |
| Primer 4 details |
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| Sequence | AGGTTTGAGATAGCAGATATTCAGT | ACCTTATGAATTCTAGCAGACTCAT | Start: 31 |
| Tm | 57.628 | 57.628 | End: 170 |
| Size | 25 | 25 | 140 |
| Primer 5 details |
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| Sequence | AGTCAAAATAAGGTTTGAGATAGCAGA | ACCTTATGAATTCTAGCAGACTCA | Start: 21 |
| Tm | 58.897 | 57.202 | End: 170 |
| Size | 27 | 24 | 150 |
