Primer Details for TRINITY_DN152838_c0_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCATTATTGCGTACTAAGCTTGT | AGAAAGGACGAGAACATTTAACCA | Start: 22 |
| Tm | 58.575 | 58.383 | End: 269 |
| Size | 24 | 24 | 248 |
| Primer 2 details |
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| Sequence | TCCATTATTGCGTACTAAGCTTGT | TGTGACAAGTAACTTGAAAACGAGT | Start: 22 |
| Tm | 58.575 | 59.36 | End: 124 |
| Size | 24 | 25 | 103 |
| Primer 3 details |
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| Sequence | ACTCGTTTTCAAGTTACTTGTCACA | AGAAAGGACGAGAACATTTAACCA | Start: 100 |
| Tm | 59.36 | 58.383 | End: 269 |
| Size | 25 | 24 | 170 |
| Primer 4 details |
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| Sequence | TGGATGTTTGTTTGAGTTTTCCA | AGAAAGGACGAGAACATTTAACCA | Start: 3 |
| Tm | 57.332 | 58.383 | End: 269 |
| Size | 23 | 24 | 267 |
| Primer 5 details |
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| Sequence | TGGATGTTTGTTTGAGTTTTCCA | TGTGACAAGTAACTTGAAAACGAGT | Start: 3 |
| Tm | 57.332 | 59.36 | End: 124 |
| Size | 23 | 25 | 122 |
