ssr_primer
Primer Details for TRINITY_DN139435_c0_g1_i1 |
|---|
| (TGG)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCACTTGGGGTCTTGGTG | TCCGCCTATGTTGACCCAAC | Start: 29 |
| Tm | 60.106 | 60.036 | End: 161 |
| Size | 20 | 20 | 133 |
| Primer 2 details |
|---|
| Sequence | AACCACTTGGGGTCTTGGTG | GGTCCAACTACAAGCGAGCT | Start: 29 |
| Tm | 60.106 | 60.037 | End: 139 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | GAGCTTGAACCACTTGGGGT | TCCGCCTATGTTGACCCAAC | Start: 22 |
| Tm | 60.179 | 60.036 | End: 161 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | TGGAGCTTGAACCACTTGGG | TCCGCCTATGTTGACCCAAC | Start: 20 |
| Tm | 60.179 | 60.036 | End: 161 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
|---|
| Sequence | GAGCTTGAACCACTTGGGGT | GGTCCAACTACAAGCGAGCT | Start: 22 |
| Tm | 60.179 | 60.037 | End: 139 |
| Size | 20 | 20 | 118 |
(GGT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCACTTGGGGTCTTGGTG | TCCGCCTATGTTGACCCAAC | Start: 29 |
| Tm | 60.106 | 60.036 | End: 161 |
| Size | 20 | 20 | 133 |
| Primer 2 details |
|---|
| Sequence | AACCACTTGGGGTCTTGGTG | GGTCCAACTACAAGCGAGCT | Start: 29 |
| Tm | 60.106 | 60.037 | End: 139 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | GAGCTTGAACCACTTGGGGT | TCCGCCTATGTTGACCCAAC | Start: 22 |
| Tm | 60.179 | 60.036 | End: 161 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | TGGAGCTTGAACCACTTGGG | TCCGCCTATGTTGACCCAAC | Start: 20 |
| Tm | 60.179 | 60.036 | End: 161 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
|---|
| Sequence | GAGCTTGAACCACTTGGGGT | GGTCCAACTACAAGCGAGCT | Start: 22 |
| Tm | 60.179 | 60.037 | End: 139 |
| Size | 20 | 20 | 118 |
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