Primer Details for TRINITY_DN135138_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGGGGAATAGACAGATTAGAATGA | TCTCCTAAACTACTTTTAATCTCCCCT | Start: 74 |
| Tm | 59.153 | 59.095 | End: 182 |
| Size | 26 | 27 | 109 |
| Primer 2 details |
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| Sequence | ACAGGGGAATAGACAGATTAGAATGA | TCTCCTAAACTACTTTTAATCTCCCC | Start: 74 |
| Tm | 59.153 | 57.913 | End: 182 |
| Size | 26 | 26 | 109 |
| Primer 3 details |
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| Sequence | ACAGGGGAATAGACAGATTAGAATGA | CTCCTAAACTACTTTTAATCTCCCCT | Start: 74 |
| Tm | 59.153 | 57.908 | End: 181 |
| Size | 26 | 26 | 108 |
| Primer 4 details |
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| Sequence | ACAGGGGAATAGACAGATTAGAATG | TCTCCTAAACTACTTTTAATCTCCCCT | Start: 74 |
| Tm | 57.928 | 59.095 | End: 182 |
| Size | 25 | 27 | 109 |
| Primer 5 details |
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| Sequence | ACAGGGGAATAGACAGATTAGAATGA | ACTACTTTTAATCTCCCCTTCTTTCT | Start: 74 |
| Tm | 59.153 | 57.779 | End: 174 |
| Size | 26 | 26 | 101 |
