ssr_primer
Primer Details for TRINITY_DN134263_c0_g1_i1 |
|---|
| (G)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGGATCTGAATGGGACGC | GCGACATGGAAATGGCATCC | Start: 36 |
| Tm | 60.179 | 59.969 | End: 136 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | ACTTGGAGACTCGCCGTTTC | GCGACATGGAAATGGCATCC | Start: 19 |
| Tm | 60.32 | 59.969 | End: 136 |
| Size | 20 | 20 | 118 |
| Primer 3 details |
|---|
| Sequence | ACTTGGAGACTCGCCGTTTC | GGCATCCGAGTTGTGAGTGA | Start: 19 |
| Tm | 60.32 | 60.037 | End: 123 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | TTCGGATCTGAATGGGACGC | CGTGTGCGACATGGAAATGG | Start: 36 |
| Tm | 60.179 | 60.179 | End: 141 |
| Size | 20 | 20 | 106 |
| Primer 5 details |
|---|
| Sequence | TTCGGATCTGAATGGGACGC | CGACATGGAAATGGCATCCG | Start: 36 |
| Tm | 60.179 | 59.691 | End: 135 |
| Size | 20 | 20 | 100 |
(AAT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGGATCTGAATGGGACGC | GCGACATGGAAATGGCATCC | Start: 36 |
| Tm | 60.179 | 59.969 | End: 136 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | ACTTGGAGACTCGCCGTTTC | GCGACATGGAAATGGCATCC | Start: 19 |
| Tm | 60.32 | 59.969 | End: 136 |
| Size | 20 | 20 | 118 |
| Primer 3 details |
|---|
| Sequence | ACTTGGAGACTCGCCGTTTC | GGCATCCGAGTTGTGAGTGA | Start: 19 |
| Tm | 60.32 | 60.037 | End: 123 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | TTCGGATCTGAATGGGACGC | CGTGTGCGACATGGAAATGG | Start: 36 |
| Tm | 60.179 | 60.179 | End: 141 |
| Size | 20 | 20 | 106 |
| Primer 5 details |
|---|
| Sequence | TTCGGATCTGAATGGGACGC | CGACATGGAAATGGCATCCG | Start: 36 |
| Tm | 60.179 | 59.691 | End: 135 |
| Size | 20 | 20 | 100 |
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