ssr_primer
Primer Details for TRINITY_DN129911_c0_g1_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCGACGGTTCCAGAGTG | GGCCAAGTCATCGACGGTTA | Start: 231 |
| Tm | 59.967 | 60.109 | End: 377 |
| Size | 20 | 20 | 147 |
| Primer 2 details |
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| Sequence | TTTCCGACGGTTCCAGAGTG | ATTGGATGTCCCATCAGCGG | Start: 231 |
| Tm | 59.967 | 60.179 | End: 395 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
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| Sequence | TGGAGTGTCTTCAAGGCGAC | ATTGGATGTCCCATCAGCGG | Start: 279 |
| Tm | 59.966 | 60.179 | End: 395 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
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| Sequence | AGTGTCTTCAAGGCGACGTT | ATTGGATGTCCCATCAGCGG | Start: 282 |
| Tm | 59.896 | 60.179 | End: 395 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
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| Sequence | TTGAGGAAGACTTGACGGCG | CACTCTGGAACCGTCGGAAA | Start: 130 |
| Tm | 60.32 | 59.967 | End: 250 |
| Size | 20 | 20 | 121 |
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