ssr_primer
Primer Details for TRINITY_DN125415_c0_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCATCCGTCCATTTTTCC | GATGAACACCCAGCCCAAGA | Start: 77 |
| Tm | 59.899 | 59.962 | End: 220 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
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| Sequence | CGGCATCCGTCCATTTTTCC | ATGAACACCCAGCCCAAGAG | Start: 77 |
| Tm | 59.899 | 59.961 | End: 219 |
| Size | 20 | 20 | 143 |
| Primer 3 details |
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| Sequence | TCTTGGGCTGGGTGTTCATC | ACACAACGGTTGCAGGAAGA | Start: 201 |
| Tm | 59.962 | 60.107 | End: 319 |
| Size | 20 | 20 | 119 |
| Primer 4 details |
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| Sequence | TGTCCAACTGATCGGCATCC | GATGAACACCCAGCCCAAGA | Start: 65 |
| Tm | 60.108 | 59.962 | End: 220 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
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| Sequence | CTCTTGGGCTGGGTGTTCAT | ACACAACGGTTGCAGGAAGA | Start: 200 |
| Tm | 59.961 | 60.107 | End: 319 |
| Size | 20 | 20 | 120 |
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