Primer Details for TRINITY_DN124032_c0_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCCTTTCAATTCAACTTACAGA | TGTGCCTCCCTGACCAATTT | Start: 9 |
| Tm | 58.299 | 59.518 | End: 175 |
| Size | 24 | 20 | 167 |
| Primer 2 details | |||
| Sequence | ACCCCTTTCAATTCAACTTACAGA | ATGTGCCTCCCTGACCAATT | Start: 9 |
| Tm | 58.299 | 59.294 | End: 176 |
| Size | 24 | 20 | 168 |
| Primer 3 details | |||
| Sequence | ACCCCTTTCAATTCAACTTACAGAA | TGTGCCTCCCTGACCAATTT | Start: 9 |
| Tm | 58.867 | 59.518 | End: 175 |
| Size | 25 | 20 | 167 |
| Primer 4 details | |||
| Sequence | AACCCCTTTCAATTCAACTTACAGA | TGTGCCTCCCTGACCAATTT | Start: 8 |
| Tm | 58.867 | 59.518 | End: 175 |
| Size | 25 | 20 | 168 |
| Primer 5 details | |||
| Sequence | AAGGACATTCAATGGGTACGAGA | ACAGACAGGGTCGTTTAAACAC | Start: 110 |
| Tm | 59.482 | 58.8 | End: 209 |
| Size | 23 | 22 | 100 |
