Primer Details for TRINITY_DN120298_c0_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTCCCTATTGCGAAATGT | ACCTTTGCTTGATGTTCTTACATTCA | Start: 107 |
| Tm | 57.582 | 59.735 | End: 252 |
| Size | 20 | 26 | 146 |
| Primer 2 details |
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| Sequence | TGCTCCCTATTGCGAAATGTTG | ACCTTTGCTTGATGTTCTTACATTCA | Start: 107 |
| Tm | 59.577 | 59.735 | End: 252 |
| Size | 22 | 26 | 146 |
| Primer 3 details |
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| Sequence | GCTCCCTATTGCGAAATGTTGT | ACCTTTGCTTGATGTTCTTACATTCA | Start: 108 |
| Tm | 59.576 | 59.735 | End: 252 |
| Size | 22 | 26 | 145 |
| Primer 4 details |
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| Sequence | GTTGCTCCCTATTGCGAAATGT | ACCTTTGCTTGATGTTCTTACATTCA | Start: 105 |
| Tm | 59.576 | 59.735 | End: 252 |
| Size | 22 | 26 | 148 |
| Primer 5 details |
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| Sequence | AGTTGCTCCCTATTGCGAAA | ACCTTTGCTTGATGTTCTTACATTCA | Start: 104 |
| Tm | 57.51 | 59.735 | End: 252 |
| Size | 20 | 26 | 149 |
