Primer Details for TRINITY_DN118053_c0_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCATGCAGATTATCCATTAGTCA | AACTCTGACAGCCCTGAACC | Start: 108 |
| Tm | 58.377 | 59.602 | End: 213 |
| Size | 24 | 20 | 106 |
| Primer 2 details |
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| Sequence | ACTGTGAAATCAGCTCTTTCTTTCA | AACTCTGACAGCCCTGAACC | Start: 55 |
| Tm | 59.175 | 59.602 | End: 213 |
| Size | 25 | 20 | 159 |
| Primer 3 details |
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| Sequence | ACTGTGAAATCAGCTCTTTCTTTCA | CAGCCCTGAACCCAAGGTAA | Start: 55 |
| Tm | 59.175 | 59.596 | End: 205 |
| Size | 25 | 20 | 151 |
| Primer 4 details |
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| Sequence | TGCATGCAGATTATCCATTAGTCA | CTGACAGCCCTGAACCCAAG | Start: 108 |
| Tm | 58.377 | 60.607 | End: 209 |
| Size | 24 | 20 | 102 |
| Primer 5 details |
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| Sequence | ACTGTGAAATCAGCTCTTTCTTTCAG | AACTCTGACAGCCCTGAACC | Start: 55 |
| Tm | 60.017 | 59.602 | End: 213 |
| Size | 26 | 20 | 159 |
