ssr_primer
Primer Details for TRINITY_DN116825_c0_g1_i1 |
|---|
| (G)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCATCCATTTTACGCGACGG | AGTGCGGCGTTCTTCCTTTA | Start: 61 |
| Tm | 59.697 | 59.966 | End: 223 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | CCATCCATTTTACGCGACGG | GCACCTTCCAATCCTTCCCA | Start: 61 |
| Tm | 59.697 | 59.96 | End: 203 |
| Size | 20 | 20 | 143 |
| Primer 3 details |
|---|
| Sequence | CATCCATTTTACGCGACGGC | AGTGCGGCGTTCTTCCTTTA | Start: 62 |
| Tm | 60.316 | 59.966 | End: 223 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | GCCATCCATTTTACGCGACG | AGTGCGGCGTTCTTCCTTTA | Start: 60 |
| Tm | 60.316 | 59.966 | End: 223 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
|---|
| Sequence | CGCCATCCATTTTACGCGAC | AGTGCGGCGTTCTTCCTTTA | Start: 59 |
| Tm | 60.316 | 59.966 | End: 223 |
| Size | 20 | 20 | 165 |
(A)31tagggttttaggagcaaaccacttaaaaaaaagagaa(G)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCATCCATTTTACGCGACGG | AGTGCGGCGTTCTTCCTTTA | Start: 61 |
| Tm | 59.697 | 59.966 | End: 223 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | CCATCCATTTTACGCGACGG | GCACCTTCCAATCCTTCCCA | Start: 61 |
| Tm | 59.697 | 59.96 | End: 203 |
| Size | 20 | 20 | 143 |
| Primer 3 details |
|---|
| Sequence | CATCCATTTTACGCGACGGC | AGTGCGGCGTTCTTCCTTTA | Start: 62 |
| Tm | 60.316 | 59.966 | End: 223 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | GCCATCCATTTTACGCGACG | AGTGCGGCGTTCTTCCTTTA | Start: 60 |
| Tm | 60.316 | 59.966 | End: 223 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
|---|
| Sequence | CGCCATCCATTTTACGCGAC | AGTGCGGCGTTCTTCCTTTA | Start: 59 |
| Tm | 60.316 | 59.966 | End: 223 |
| Size | 20 | 20 | 165 |
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