ssr_primer
Primer Details for TRINITY_DN109678_c288_g1_i1 |
|---|
| (T)17ccttaaa(T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGCCCTTCTCATGCAATCA | CGACTTCCTTTGGGGGTTGT | Start: 3 |
| Tm | 59.717 | 60.179 | End: 266 |
| Size | 21 | 20 | 264 |
| Primer 2 details |
|---|
| Sequence | CAGCCCTTCTCATGCAATCA | CGACTTCCTTTGGGGGTTGT | Start: 4 |
| Tm | 58.241 | 60.179 | End: 266 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
|---|
| Sequence | TCAGCCCTTCTCATGCAATCA | CCGACTTCCTTTGGGGGTTG | Start: 3 |
| Tm | 59.717 | 60.894 | End: 267 |
| Size | 21 | 20 | 265 |
| Primer 4 details |
|---|
| Sequence | TCAGCCCTTCTCATGCAATC | CGACTTCCTTTGGGGGTTGT | Start: 3 |
| Tm | 57.952 | 60.179 | End: 266 |
| Size | 20 | 20 | 264 |
| Primer 5 details |
|---|
| Sequence | TCAGCCCTTCTCATGCAATCAT | CGACTTCCTTTGGGGGTTGT | Start: 3 |
| Tm | 60.092 | 60.179 | End: 266 |
| Size | 22 | 20 | 264 |
(A)11caaaaataagactaccattaat(A)13caaaaagaaaacaaaacaaaccaagaaaaaaacaacccccaaaggaagtcggcccactgggtgggcgggggc(A)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGCCCTTCTCATGCAATCA | CGACTTCCTTTGGGGGTTGT | Start: 3 |
| Tm | 59.717 | 60.179 | End: 266 |
| Size | 21 | 20 | 264 |
| Primer 2 details |
|---|
| Sequence | CAGCCCTTCTCATGCAATCA | CGACTTCCTTTGGGGGTTGT | Start: 4 |
| Tm | 58.241 | 60.179 | End: 266 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
|---|
| Sequence | TCAGCCCTTCTCATGCAATCA | CCGACTTCCTTTGGGGGTTG | Start: 3 |
| Tm | 59.717 | 60.894 | End: 267 |
| Size | 21 | 20 | 265 |
| Primer 4 details |
|---|
| Sequence | TCAGCCCTTCTCATGCAATC | CGACTTCCTTTGGGGGTTGT | Start: 3 |
| Tm | 57.952 | 60.179 | End: 266 |
| Size | 20 | 20 | 264 |
| Primer 5 details |
|---|
| Sequence | TCAGCCCTTCTCATGCAATCAT | CGACTTCCTTTGGGGGTTGT | Start: 3 |
| Tm | 60.092 | 60.179 | End: 266 |
| Size | 22 | 20 | 264 |
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