Primer Details for TRINITY_DN109678_c236_g1_i1 | |||
|---|---|---|---|
| (T)18aactacctcaaataggtaacgacttatttttcttctgtttttttt(C)16aaaaaaatcaagctttcgccccccccctc(T)13ctttttttcttg(T)13ctttttttgttg(T)13: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAATCAAGCTTTCGCCCCC | ACAAAAACCACAAAAACGAAACGA | Start: 82 |
| Tm | 59.392 | 59.256 | End: 193 |
| Size | 20 | 24 | 112 |
| Primer 2 details | |||
| Sequence | AAAATCAAGCTTTCGCCCCC | ACAAAAACCACAAAAACGAAACG | Start: 82 |
| Tm | 59.392 | 57.992 | End: 193 |
| Size | 20 | 23 | 112 |
| Primer 3 details | |||
| Sequence | AAAAATCAAGCTTTCGCCCCC | ACAAAAACCACAAAAACGAAACGA | Start: 81 |
| Tm | 59.998 | 59.256 | End: 193 |
| Size | 21 | 24 | 113 |
| Primer 4 details | |||
| Sequence | AAAATCAAGCTTTCGCCCCC | ACAAAAACCACAAAAACGAAACGAA | Start: 82 |
| Tm | 59.392 | 59.766 | End: 193 |
| Size | 20 | 25 | 112 |
| Primer 5 details | |||
| Sequence | AAAATCAAGCTTTCGCCCCC | AACAAAAACCACAAAAACGAAACGA | Start: 82 |
| Tm | 59.392 | 59.766 | End: 194 |
| Size | 20 | 25 | 113 |
