Primer Details for TRINITY_DN109678_c219_g1_i1 |
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| (A)11tatcaaaaaaaaaccaataaagcaaataaaaacg(A)12ttaaaaaaaaaccaaaaaaaaactaaaagtgaaaag(A)12taaaaagat(A)16taaaaacag(A)10g(A)11ccaaaaacaaaaactgacaaacaaaaaaccccacc(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGGGGGAAGCGAGTAAAT | TGTTTGTCAGTTTTTGTTTTTGGT | Start: 24 |
| Tm | 59.536 | 57.073 | End: 240 |
| Size | 20 | 24 | 217 |
| Primer 2 details |
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| Sequence | CGAGGGGGAAGCGAGTAAAT | TGTTTGTCAGTTTTTGTTTTTGGTT | Start: 24 |
| Tm | 59.536 | 57.678 | End: 240 |
| Size | 20 | 25 | 217 |
| Primer 3 details |
|---|
| Sequence | CGAGGGGGAAGCGAGTAAAT | TTGTTTGTCAGTTTTTGTTTTTGGT | Start: 24 |
| Tm | 59.536 | 57.678 | End: 241 |
| Size | 20 | 25 | 218 |
| Primer 4 details |
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| Sequence | CGAGGGGGAAGCGAGTAAAT | TGTTTGTCAGTTTTTGTTTTTGGTTT | Start: 24 |
| Tm | 59.536 | 58.238 | End: 240 |
| Size | 20 | 26 | 217 |
| Primer 5 details |
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| Sequence | CGAGGGGGAAGCGAGTAAAT | TTGTTTGTCAGTTTTTGTTTTTGGTT | Start: 24 |
| Tm | 59.536 | 58.238 | End: 241 |
| Size | 20 | 26 | 218 |
