ssr_primer
Primer Details for TRINITY_DN109678_c215_g1_i1 |
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| (C)12gcccgccacaaccccccccctcccttctctcatttctctgcgcgcttttttcgcccccttccccttcctcccctaa(C)20tcccg(C)19aaaccaacaaaaacaaaaaaaatatcacaacaacc(A)11caaaaaaaataaactacaaataaaaaattaaaaat(A)10cccgaaaaat(A)16gcataaacacctaac(A)15tatatagaaaaagggatcacaaaaagggggtagagggcctgggaaggggaaaaaaaaagcttcgt(A)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTTCCCCTTCCTCCCCTAA | GGCCCTCTACCCCCTTTTTG | Start: 73 |
| Tm | 59.954 | 60.324 | End: 322 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
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| Sequence | CCTTCCCCTTCCTCCCCTAA | GCCCTCTACCCCCTTTTTGT | Start: 73 |
| Tm | 59.954 | 59.592 | End: 321 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
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| Sequence | CCTTCCCCTTCCTCCCCTAA | AGGCCCTCTACCCCCTTTTT | Start: 73 |
| Tm | 59.954 | 60.48 | End: 323 |
| Size | 20 | 20 | 251 |
| Primer 4 details |
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| Sequence | TTCCCCTTCCTCCCCTAACC | GGCCCTCTACCCCCTTTTTG | Start: 75 |
| Tm | 60.253 | 60.324 | End: 322 |
| Size | 20 | 20 | 248 |
| Primer 5 details |
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| Sequence | TTCCCCTTCCTCCCCTAACC | GCCCTCTACCCCCTTTTTGT | Start: 75 |
| Tm | 60.253 | 59.592 | End: 321 |
| Size | 20 | 20 | 247 |
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