Primer Details for TRINITY_DN109678_c205_g1_i1 | |||
|---|---|---|---|
| (A)16ttttaaactgaattattttaaaaatcttccgatttt(A)10g(A)11ttttttaaaaaaatttcggcccaaatcaaaatc(A)17: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAAATTTCGGCCCAAATCAAAATCA | AGGACCCTACTGCAAAAAGTCC | Start: 82 |
| Tm | 59.736 | 60.225 | End: 181 |
| Size | 26 | 22 | 100 |
| Primer 2 details | |||
| Sequence | AAAAATTTCGGCCCAAATCAAAAT | AGGACCCTACTGCAAAAAGTCC | Start: 82 |
| Tm | 57.353 | 60.225 | End: 181 |
| Size | 24 | 22 | 100 |
| Primer 3 details | |||
| Sequence | AAAAATTTCGGCCCAAATCAAAATC | AGGACCCTACTGCAAAAAGTCC | Start: 82 |
| Tm | 58.323 | 60.225 | End: 181 |
| Size | 25 | 22 | 100 |
| Primer 4 details | |||
| Sequence | AATTTCGGCCCAAATCAAAATCA | AAAAGGACCCTACTGCAAAAAGT | Start: 85 |
| Tm | 58.096 | 58.713 | End: 184 |
| Size | 23 | 23 | 100 |
| Primer 5 details | |||
| Sequence | AAATTTCGGCCCAAATCAAAATCA | AAAGGACCCTACTGCAAAAAGTC | Start: 84 |
| Tm | 58.688 | 59.11 | End: 183 |
| Size | 24 | 23 | 100 |
