Primer Details for TRINITY_DN109678_c157_g1_i1 |
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| (A)11caaaaaaaaataac(A)11taaaaaaaaacaacaactcatcttcaacaaatacaacc(A)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCCTTTAACAAATAGCTTCCAGA | TGGTTGTATTTGTTGAAGATGAGTTGT | Start: 30 |
| Tm | 58.22 | 59.932 | End: 185 |
| Size | 25 | 27 | 156 |
| Primer 2 details |
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| Sequence | ACTCCTTTAACAAATAGCTTCCAGAG | TGGTTGTATTTGTTGAAGATGAGTTGT | Start: 30 |
| Tm | 59.118 | 59.932 | End: 185 |
| Size | 26 | 27 | 156 |
| Primer 3 details |
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| Sequence | ACTCCTTTAACAAATAGCTTCCAGA | TGGTTGTATTTGTTGAAGATGAGTTG | Start: 30 |
| Tm | 58.22 | 58.575 | End: 185 |
| Size | 25 | 26 | 156 |
| Primer 4 details |
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| Sequence | ACTCCTTTAACAAATAGCTTCCAGA | GGTTGTATTTGTTGAAGATGAGTTGT | Start: 30 |
| Tm | 58.22 | 58.574 | End: 184 |
| Size | 25 | 26 | 155 |
| Primer 5 details |
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| Sequence | AACTCCTTTAACAAATAGCTTCCAGA | TGGTTGTATTTGTTGAAGATGAGTTGT | Start: 29 |
| Tm | 58.769 | 59.932 | End: 185 |
| Size | 26 | 27 | 157 |
