Primer Details for TRINITY_DN109059_c0_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGAGCAAATGATTTCTTTGACGT | AAACCTGACCAGCCGAACAT | Start: 27 |
| Tm | 59.761 | 59.89 | End: 166 |
| Size | 25 | 20 | 140 |
| Primer 2 details |
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| Sequence | TGAGAGCAAATGATTTCTTTGACGT | CATGAAACCTGACCAGCCGA | Start: 27 |
| Tm | 59.761 | 60.322 | End: 170 |
| Size | 25 | 20 | 144 |
| Primer 3 details |
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| Sequence | TGAGAGCAAATGATTTCTTTGACGT | TCATGAAACCTGACCAGCCG | Start: 27 |
| Tm | 59.761 | 60.322 | End: 171 |
| Size | 25 | 20 | 145 |
| Primer 4 details |
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| Sequence | TGAGAGCAAATGATTTCTTTGACGT | ATGAAACCTGACCAGCCGAA | Start: 27 |
| Tm | 59.761 | 59.6 | End: 169 |
| Size | 25 | 20 | 143 |
| Primer 5 details |
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| Sequence | GTGAGAGCAAATGATTTCTTTGACG | AAACCTGACCAGCCGAACAT | Start: 26 |
| Tm | 59.433 | 59.89 | End: 166 |
| Size | 25 | 20 | 141 |
