ssr_primer
Primer Details for TRINITY_DN105658_c0_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCGACAATCTGAGAAAGC | TGGACCCAGCACCATTTCTC | Start: 122 |
| Tm | 59.628 | 59.962 | End: 345 |
| Size | 20 | 20 | 224 |
| Primer 2 details |
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| Sequence | GAAAATATCGCACGTCGCCC | GCTTTCTCAGATTGTCGCGG | Start: 0 |
| Tm | 60.042 | 59.628 | End: 141 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
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| Sequence | CCGCGACAATCTGAGAAAGC | TAACAAGGTGTGGACCCAGC | Start: 122 |
| Tm | 59.628 | 59.89 | End: 355 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
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| Sequence | CCGCGACAATCTGAGAAAGC | GTGGACCCAGCACCATTTCT | Start: 122 |
| Tm | 59.628 | 60.251 | End: 346 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
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| Sequence | AAAATATCGCACGTCGCCCT | GCTTTCTCAGATTGTCGCGG | Start: 1 |
| Tm | 60.461 | 59.628 | End: 141 |
| Size | 20 | 20 | 141 |
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